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Prinsip pembuatan susu bubuk adalah menguapkan sebanyak mungkin kandungan air susu dengan cara pemanasan (pengeringan). Terdapat empat jenis susu bubuk berdasarkan cara pengolahannya, yaitu:
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Di dalam susu terdapat enzim peroksidase, katalase, fosfatase dan lipase. Peroksidase dan fosfatase dapat dijadikan sebagai indikator kecukupan pasteurisasi susu karena kedua enzim ini akan rusak pada suhu pasteurisasi. Sedangkan lipase dapat menyebabkan kerusakan pada susu.
Berbagai senyawa kimia yang terkandung di dalam susu selain menentukan sifat kimiawinya, juga berpengaruh terhadap sifat fisik susu. Secara indrawi susu tampak sebagai cairan berwarna putih sedikit kekuningan atau kebiruan serta mempunyai rasa gurih khas sedikit manis.
Namun proses ini tidak dapat mematikan bakteri bersifat termoresisten alias tahan pada suhu tinggi. Kontaminasi pseteurisasi terjadi melalui peralatan pengolahan dan kemasan yang digunakan. Kemasan yang sering digunakan pseteurisasi adalah gelas, botol, plastik serta kemasan fleksibel berbentuk pouch.